94. Solving a chemistry problem

Product description

The absorbance of 1.00 *10 -4 mol/l of a solution of a certain substance was measured at various wavelengths in a cuvette with an absorbing layer thickness of 0.50 cm. Using the data below, plot the absorption spectrum of the test solution in the coordinates absorbance A - wavelength λ, nm.

Select the analytical wavelength at which the greatest accuracy and sensitivity of determining the substance by the spectrophotometric method is achieved, and at the selected wavelength, calculate the molar extinction coefficient of this substance in solution.

Wavelength, nm Optical density

350 0.090

355-

360 0.085

365-

370 0.075

380 0.085

390 0.110

395-

400 0.190

410 0.325

420 0.460

430 0.600

435 0.635

440 0.630

450 0.575

460 0.475

470 0.360

480 0.257

490 0.212

500 0.165

Additional information

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