94. Solving a chemistry problem

Product description

• The optical density of 1.00 * 10–4 mol / l of a solution of a certain substance was measured at various wavelengths in a cuvette with an absorbing layer thickness of 5.00 cm (option a), 1.00 cm (options b, d, e), 0.50 cm (option c). Using the data below, construct the absorption spectrum of the test solution in the coordinates optical density A - wavelength λ, nm.

Select the analytical wavelength at which the greatest accuracy and sensitivity of determining the substance by the spectrophotometric method is achieved, and at the selected wavelength, calculate the molar extinction coefficient of this substance in solution.

Wavelength, nm Optical density

Option

b

350 0.784

355-

360 0.392

365 0.366

370 0.376

380 0.455

390 0.523

395 0.540

400 0.537

410 0.454

420 0.284

430 0.193

435-

440 0.183

450 0.157

460 0.088

470 0.044

480 0.018

490 0.009

500 0.003

Additional information

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